Short description of project
Human induced Pluripotent Stem Cells (iPSCs) are derived from somatic cells by overexpressing specific factors through reprogramming. iPSCs have great potentials in drug screening, disease modeling and patient specific cell-based therapies, but the cost and manpower required to generate iPSCs are usually overwhelming for regular labs. At the same time, the iPSC derivation conditions often limit their application potentials if undefined components or animal products are used in the process. It would be a great technological progress if iPSCs could be generated under clinically relevant conditions with acceptable cost in high throughput fashion. We previously developed a next generation stem cell culture system, E8 medium system, for human iPSC derivation and expansion in low throughput platform. I propose to build a high throughput platform to increase production efficiency, lower cost, and generate large numbers of high quality human iPSCs in clinically relevant settings. At the same time, we will develop novel assays to evaluate quality of newly derived iPSCs. This project will involve the optimization of existing methods and the development of novel technologies. If successful, it will become a fundamental platform in the stem cell field and will improve the access to iPSCs for the stem cell communities.
Information of Offered Internship
Level of Internship Hours per Month
Level 3 (60 hrs)
Commencement Month
June
Duration
6 months
Internship requirements: i.e. work, practice and training
1. Help differentiate stem cells to specific lineages,
2. Setup Assays to analyze differentiated cells,
3. Help lab organization
4. Help process some data in the lab
2. Setup Assays to analyze differentiated cells,
3. Help lab organization
4. Help process some data in the lab