Short description of project
Diabetes mellitus is one of ten leading underlying causes of mortality in Macau. Patients with diabetic mellitus
suffer from complications including diabetic foot ulcers, which can result in lower limb amputations. The
methyltransferase SET7/9 interacts and methylates HIF-1α, triggering the breakdown of HIF-1α in the nucleus.
Elevation of SET7/9-mediated methylation and consequent destabilization of HIF-1α induced by hyperglycemia
contributes to the pathophysiological mechanisms of diabetic wounds. Thus, stabilization of HIF-1α by blocking
the interaction of SET7/9 and HIF-1α could be a potential strategy to improve wound healing, especially in diabetes.
In this study, we aim to identify inhibitors of the SET7/9–HIF-1α interaction from libraries of bioactive compounds or in-house metal-based compounds for the treatment of diabetic wounds.
suffer from complications including diabetic foot ulcers, which can result in lower limb amputations. The
methyltransferase SET7/9 interacts and methylates HIF-1α, triggering the breakdown of HIF-1α in the nucleus.
Elevation of SET7/9-mediated methylation and consequent destabilization of HIF-1α induced by hyperglycemia
contributes to the pathophysiological mechanisms of diabetic wounds. Thus, stabilization of HIF-1α by blocking
the interaction of SET7/9 and HIF-1α could be a potential strategy to improve wound healing, especially in diabetes.
In this study, we aim to identify inhibitors of the SET7/9–HIF-1α interaction from libraries of bioactive compounds or in-house metal-based compounds for the treatment of diabetic wounds.
Information of Offered Internship
Level of Internship Hours per Month
Level 1 - 40 hours
Commencement Month
July
Duration
5 Months
Internship requirements: i.e. work, practice and training
Internship Requirements:
1. Will conduct literature search.
2. Will prepare materials and buffer for gel electrophoresis and cell culture.
3. Will handle simple biological experiments, such as DNA and protein extractions.
4. Will collect experimental data.
1. Will conduct literature search.
2. Will prepare materials and buffer for gel electrophoresis and cell culture.
3. Will handle simple biological experiments, such as DNA and protein extractions.
4. Will collect experimental data.